primary anti noxa rabbit polyclonal antibody  (Abcam)

Journal: Oncogene

Article Title: SOX30, a novel epigenetic silenced tumor suppressor, promotes tumor cell apoptosis by transcriptional activating p53 in lung cancer

doi: 10.1038/onc.2014.370

Figure Lengend Snippet: SOX30 accelerates expression of p53 signaling pathway-related genes. ( a ) Ectopic expression of SOX30 significantly ( P <0.01) enhances endogenous expression of P53, P21, BAX and PMAIP1 in A549 and H460 cells. The mRNA expression was examined by qRT–PCR, and ACTIN was used as an internal control. * P <0.05; ** P <0.01. ( b ) The protein levels of P53, P21, BAX and PMAIP1 were detected by WB after restoration of SOX30 in A549 and H460 cells. ACTIN was used as an internal control. ( c , d ) The mRNA and protein expression levels of SOX30 and P53 in xenograft tumors were examined by qRT–PCR and WB. ACTIN was used as an internal control. ( e ) SOX30 increases the number of terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL)-positive H460 cells in xenograft tumors. Two (1 and 2) representative images were randomly selected from each sample (H460-vector and H460-SOX30). The arrows indicate apoptotic cells.

Article Snippet: Primary antibodies were SOX30 rabbit polyclonal antibody (1:1000; Santa Cruz Biotechnology), p53 mouse polyclonal antibody (1:1000; Santa Cruz Biotechnology), BAX rabbit polyclonal antibody (1:1000; Santa Cruz Biotechnology), P21 rabbit polyclonal antibody (1:1000; Cell Signaling Technology, Boston, MA, USA) and PMAIP1 (NOXA) rabbit polyclonal antibody (1:1000; Santa Cruz Biotechnology).

Techniques: Expressing, Quantitative RT-PCR, End Labeling, TUNEL Assay, Plasmid Preparation